Skip to main content
. 2014 Dec 9;156(2):475–487. doi: 10.1210/en.2014-1427

Figure 3.

Figure 3.

NEFA plays a critical role on the activated senescence pathway in osteoblastic cells, whereas isoflavone prevented it. A, OB6 cells treated with vehicle (control), NEFA, isoflavone, and their combination were stained with SA-β-gal. NEFA-treated cells showed more SA-β-gal-positive blue cells. B, ELISA for SA-β-gal activity in total proteins isolated from OB6 cells with 4 different treatments. C, Real-time PCR analyses for p21 mRNA expression after OB6 cells were treated with NEFA, isoflavone, and their combination for 3 days. D, Real-time PCR analyses for p53 mRNA expression after OB6 cells treated with NEFA, isoflavone, and their combination for 3 days. E, Western blots for p53, p21, PPARγ, and Sirt1 protein expression in OB6 cells in triplicate after treatment with NEFA, isoflavone, or their combination for 3 days. F, The bar graphs represent the ratio of intensity of the band of p53, p21, PPARγ, and Sirt1 protein expression normalized to β-actin protein for 3 samples each from 4 different treatments using Quantity One software. Data are expressed as means ± SD. Means with different letters differ significantly from each other at P < .05 by two-way ANOVA followed by the Tukey-Kramer post hoc test for pairwise comparisons.