Skip to main content
. 2014 Dec 9;156(2):475–487. doi: 10.1210/en.2014-1427

Figure 4.

Figure 4.

NEFA facilitated PPARγ binding to p21/p53 promoter in OB6 cells, and isoflavone blocked it. A, Luciferase activity in OB6 cells transfected with a PPRE-luc reporter construct and treated with NEFA, isoflavone (Isof), and their combination for 24 and 48 hours. ULR, unit of luminescence to renilla; Luc, luciferase; Ren, Renilla. B, Schematic diagram of the p21 and p53 gene promoters and design for 4 sets of primers from each p21 and p53 gene promoter. C and D, ChIP (IP) of 4 sets of possible mouse p21 and p53 enhancer elements by specific anti-PPARγ antibody in OB6 cells after treatment with NEFA, isoflavones, and their combination for 24 hours. Bars represent means ± SD in triplicate. Means with different letters differ significantly from each other at P < .05.