Table 1.
Effects of Genotype and Feeding on the Ratio of pS6 to Total S6 in Different Tissues From DW (Mut) and GHR−/− (Mut) Mice Compared With Control Mice (WT)
| Tissue | Pro. | Genot. | WT/Mut (Fasted) | WT/Mut (Fed) | Fed/Fasted (Mutant) | Fed/fasted (WT) | ANOVA |
|---|---|---|---|---|---|---|---|
| Liver | pS6 | Snell | 2.2b | 2.4b | 10.4b | 11.6c | G + N |
| GHR−/− | 1.4a | 2.7c | 5.6c | 11.1c | G + N + Int | ||
| Skeletal muscle | pS6 | Snell | 2.0b | 2.0b | 3.1b | 3.1c | G + N |
| GHR−/− | 3.2a | 3.0c | 3.3a | 4.3c | G + N | ||
| Heart | pS6 | Snell | 2.3c | 4.1c | 2.2a | 3.9c | G + N |
| GHR−/− | 1.4 | 1.6b | 2.8b | 3.2c | G + N | ||
| Kidneys | pS6 | Snell | 1.6a | 2.1a | 3.2b | 4.7c | G + N |
| GHR−/− | 1.4b | 1.9b | 4.3b | 6.0c | G + N |
Values represent the mean fold change between compared groups. Unpaired t tests were used on untransformed data to compare individual groups. Two-way ANOVA was used to test the genotype (G, P < 0.05), nutritional (N, P < 0.05) and interaction (Int, P < 0.05) effects using log-transformed values.
a
P < 0.05.
b
P < 0.01.
c
P < 0.001.