Figure 1.

Diagram of TRα TALEN design. A, Binding regions for left and right arms of TRα TALEN (bold nucleotides) flank a spacer region in which a double-stranded break (black triangle) catalyzed by FokI nuclease domains is repaired by nonhomologous end joining. Zinc finger domains, amino acids, and exon boundaries (E4, E5, E6) are indicated. B, TRα is composed of nine exons (E1–9), and the targeted region is within exon 5 (E5), part of the coding region for the DNA binding domain (DBD). Black bars in E1 and E9 represent 5′ and 3′ untranslated regions, respectively. A/B, C, D, E/F, nuclear receptor protein domains, LBD, ligand binding domain.