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Primer sequence of ALP, OCN, OPN, and GAPDH used for Q-PCR
| Gene | Gene ID | Forward/reverse |
|---|---|---|
| ALP | NM_000478 | TGCAGTACGAGCTGAACAGG/GTCAATTCTGCCTCCTTCCA |
| OCN | NM_199173 | GGCAGCGAGGTAGTGAAGA/TCAGCCAACTCGTCACAGTC |
| OPN | NM_001040060 | TCAGCTGGATGACCAGAGTG/TTGGGGTCTACAACCAGCAT |
| GAPDH | NM_002046 | TGTTCGTCATGGGTGTGAAC/GTCTTCTGGGTGGCAGTGAT |
Abbreviations: ALP, alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Q-PCR, quantitative polymerase chain reaction.
