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Active zones at representative neuromuscular junctions of wandering stage III female larvae. (A) control line. (B) stcP line. Left panels: NMJs are probed with anti-BRP (Bruchpilot), red color. Middle panels: NMJs are probed with anti-HRP (HorseRedish Peroxidase), green color. Right panels: merge. Enlarged parts of NMJs are shown in white frames. White arrows indicate red grains corresponding to active zones that were counted to characterize synaptic activity.
