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. 2014 Dec 28;6(12):1076–1093. doi: 10.18632/aging.100712

Table 4. stc transcript amounts in control, mutant and revertant lines.

Source of RNA Line No of experiments Mean (s. e.), arbitrary units P values for comparisons with stcP line
t test Kruskal-Wallis test
Embryos, 0-12 hours Control 1 0.320 (0.02)*
stcP 1 0.224 (0.01)*
Embryos, 12-18 hours Control 1 0.333 (0.02)*
stcP 1 0.133 (0.03)*
Embryos, 0-18 hours Control 4 0.294 (0.003) 0.0033 0.0209
stcP 4 0.140 (0.001)
rev3 4 0.444 (0.100) 0.0273 0.0833
Embryos, 0-18 hours Control 4 0.894 (0.138) 0.0073 0.0209
stcP 4 0.286 (0,066)
rev3 4 0.869 (0.097) 0.0025 0.0209
Female larvae, III stage Control 5 0.459 (0.040) 0.5493 0.6242
stcP 4 0.407 (0.077)
Female larvae, III stage Control 5 0.238 (0.029) 0.3305 0.2207
stcP 4 0.191 (0.035)
Females, 5-6 hours Control 5 0.318 (0.021) 0.7415 0.8065
stcP 5 0.300 (0.045)
Unmated females, 20 days Control 4 0.565 (0.110) 0.3977 0.5637
stcP 4 0.449 (0.063)
Unmated females, 20 days, RB Control 2 0.401 (0.083) 0.8511 1
stcP 2 0.433 (0.123)
Unmated females, 60 days Control 5 0.286 (0.064) 0.4960 0.6015
stcP 5 0.376 (0.110)
Mated females, 20 days Control 4 0.658 (0.017) 0.5074 0.4624
stcP 5 0.630 (0.034)
Mated females, 60 days Control 5 0.834 (0.126) 0.4645 0.6242
stcP 4 1.048 (0.268)
Ovaries, unmated females, 20 days Control 2 0.757 (0.197) 0.9851 1
stcP 2 0.747 (0.054)
Ovaries, unmated females, 50 days Control 2 0.782 (0.292) 0.9232 0.4386
stcP 2 0.707 (0.275)
Ovaries, mated females, 20 days Control 2 1.019 (0.329) 0.8572 0.4386
stcP 2 1.242 (0.284)
Ovaries, mated females, 50 days Control 2 0.749 (0.202) 0.9879 0.4386
stcP 2 0.738 (0.194)
Brain, female larvae, III stage Control 5 0.251 (0.090) 0.7809 0.7540
stcP 5 0.221 (0.069)
Heads, females, 5-6 hours Control 4 2.867 (0.420) 0.8848 0.3272
stcP 5 3.074 (0.515)
Unmated males, 20 days Control 4 0.211 (0.036) 0.3113 0.2482
stcP 4 0.162 (0.026)
Unmated males, 20 days, RB Control 2 0.207 (0.010) 0.1618 0.1213
stcP 2 0.154 (0.019)
Unmated males, 60 days Control 2 0.513 (0.165) 0.7832 0.4386
stcP 2 0.441 (0.162)

Standard errors were calculated from the variance among biological replicates.

*

Standard errors were calculated from the variance among technical replicates.

Significant P-values are in bold case.

The amount of RA and RB stc transcripts was measured in all cases except when sole RB transcript is specially indicated