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. 2014 Aug 1;3(6):1544–1552. doi: 10.1002/cam4.309

Figure 5.

Figure 5

The miR-124-5p mimic inhibited SMC4 mRNA expression in WiDr and COLO201 cells. The synthetic miR-124-5p microRNA mimic or SMC4 siRNA inhibited SMC4 mRNA expression in WiDr (A) and COLO201 (B) cells. The synthetic microRNA mimic or siRNA were transfected using Lipofectamine 2000 transfection agent. Cells were harvested for 72 h. Values represent the relative ratio of target gene per GAPDH, mean ± SEM, to the control from eight independent experiments. The control group was transfected with negative control RNA (AllStars Negative Control siRNA, Qiagen). miR-124-5p: the mimic of miR-124-5p was transfected; SMC4 siRNA: synthetic SMC4 siRNA was transfected. Statistical analysis was performed using the Tukey–Kramer test; **P < 0.01, ***P < 0.001.