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. 2014 Aug 30;3(6):1605–1614. doi: 10.1002/cam4.329

Figure 1.

Figure 1

Downregulation of Bim in Tax-transformed CTLL-2 cells. (A, B) Cell lysates were prepared from CTLL-2 cells cultured in the presence or absence of IL-2 for 18 h and from Tax-transformed CTLL-2 cells cultured without IL-2, and the levels of Bim, p-Erk, Tax, and α-tubulin were analyzed by western blotting using the corresponding antibodies. Band intensities of Bim and p-Erk were quantified and normalized to α-tubulin and Erk, respectively. (C) Total RNA was prepared from CTLL-2 cells cultured in the presence or absence of IL-2 for 18 h and from Tax-transformed CTLL-2 cells cultured without IL-2. The amount of Bim transcript was measured by real-time PCR and normalized to the amount of GAPDH RNA.