Figure 3.

Splenic lymphocyte populations increase steadily after birth. Spleen cells were isolated and subjected to flow cytometry analysis. CD3, immunoglobulin (Ig)D and CD11b were used to identify T and B lymphocytes and myeloid cells, respectively. (a) T, B and myeloid cell populations at various times after birth, six mice per group. *,# = P < 0·05 compared to the same cell population on postnatal day 0–5. (b) Representative histograms of CD19 and CD3 staining of splenocytes from mice at three different ages.