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. 2015 Jan 2;179(2):210–219. doi: 10.1111/cei.12456

Figure 1.

Figure 1

The binding immunoglobulin protein (BiP) transgene induced biologically active BiP protein production: (a) 5 × 104 human embryonic kidney (HEK) 293T cells were transfected with empty vector or pgBiP. Serial BiP concentration in culture supernatants were measured at 24, 48, 72 and 96 h after transfection by enzyme-linked immunosorbent assay (ELISA). (b) Serial dilutions of mBiP or recombinant human (rhu)BiP were used to stimulate 1 × 106 cultured human peripheral blood mononuclear cells (PBMCs). The production of cytokines, interleukin (IL)-10 and tumour necrosis factor (TNF)-α, induced by the addition of mBiP or recombinant human (rhu) BiP protein, was measured at 24 h. (c) Mixed splenocytes/lymph nodes (2·5 × 106) from dilute brown non-Agouti/1 (DBA/1) mice were stimulated with 20 μg of rhuBiP or mBiP. Induced IL-10 and TNF-α were measured at 24 h. Significance was determined by Student's unpaired t-test. *P < 0·05.