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. 2015 Jan 2;179(2):344–353. doi: 10.1111/cei.12458

Figure 6.

Figure 6

Interleukin (IL)-17A induces serum amyloid A (SAA) production from keratinocytes. (a) The neonatal foreskin-isolated normal primary keratinocytes were cultured for 2, 6 and 24 h in the presence of IL-17A at the indicated concentrations (10, 50 and 200 ng/ml), the level of SAA mRNA in the cells was analysed by real-time transcription–polymerase chain reaction (RT–PCR) and the data were normalized for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Gene expression is graphed as mean fold induction over medium control. The data represent the mean ± standard error of the mean (s.e.m.) of three experiments with similar results (*P < 0·05; **P < 0·01). (b) Normal primary keratinocytes were treated with 200 ng/ml IL-17A for 24 h, then SAA protein was measured by immunofluorescence. Scale bars represent 100 μm. (c) Normal primary keratinocytes were treated with 200 ng/ml IL-17A in the presence of neutralizing anti-IL-17RA antibodies. After 48 h, supernatants were harvested and tested for SAA by enzyme-linked immunosorbent assay (ELISA). The values indicate the mean ± s.e.m. of three experiments (*P < 0·05; **P < 0·01).