Table 4.

Summary of molecular diversity indices of COI,CytB,wgl,elongation factor 1α genes, mitochondrial DNA (combined COI and CytB), and nuclear DNA (nDNA); sample size (n), number of haplotypes (no.), number of variable sites (S), average number of nucleotide differences (k), haplotype diversity (h), and nucleotide diversity (π) with standard deviation (SD). Nuclear genes have been doubled for analysis by the program DNAsp to avoid ambiguous sites, as DNAsp cannot analyze diploide genetic information

Gene	Species	n	no.	S	k	h (±SD)	π (±SD)
COI	M. jurtina	47	20	40	6.29	0.834 ± 0.048	0.01065 ± 0.00185
	M. nurag	19	8	33	10.60	0.871 ± 0.044	0.01849 ± 0.00213
	M. megala	5	1	–	–	–	–
	M. cypricola	12	6	26	8.86	0.848 ± 0.074	0.01497 ± 0.00461
	M. chia	16	5	15	2.26	0.65 ± 0.108	0.00474 ± 0.00283
	M. telmessia	11	7	30	6.47	0.873 ± 0.089	0.00985 ± 0.00491
	M. halicarnassus	8	4	7	2.61	0.750 ± 0.139	0.00397 ± 0.00114
	Lineage A	31	14	17	3.71	0.890 ± 0.036	0.00627 ± 0.00059
	Lineage B	87	23	42	7.27	0.864 ± 0.025	0.01585 ± 0.00138
	All samples	118	37	48	8.84	0.919 ± 0.015	0.01926 ± 0.00099
CytB	M. jurtina	29	14	30	5.48	0.894 ± 0.040	0.01305 ± 0.00404
	M. nurag	12	7	32	11.67	0.909 ± 0.056	0.02804 ± 0.00382
	M. megala	5	1	–	–	–	–
	M. cypricola	14	8	25	7.82	0.868 ± 0.068	0.01867 ± 0.00563
	M. chia	7	4	24	8.48	0.714 ± 0.181	0.02173 ± 0.00831
	M. telmessia	16	7	24	4.00	0.792 ± 0.089	0.00952 ± 0.00490
	M. halicarnassus	8	5	6	1.79	0.786 ± 0.151	0.00413 ± 0.00120
	All samples	91	33	43	10.54	0.906 ± 0.021	0.02703 ± 0.00106
wgl	M. jurtina	30 (60)	11	8	1.47	0.774 ± 0.041	0.00432 ± 0.00044
	M. nurag	12 (24)	13	11	2.57	0.906 ± 0.046	0.00754 ± 0.00095
	M. megala	5 (10)	7	8	3.62	0.933 ± 0.062	0.00899 ± 0.00106
	M. cypricola	11 (22)	6	7	1.05	0.671 ± 0.077	0.00281 ± 0.00066
	M. chia	14 (28)	9	5	1.83	0.831 ± 0.051	0.00537 ± 0.00051
	M. telmessia	14 (28)	6	3	1.18	0.741 ± 0.067	0.00348 ± 0.00040
	M. halicarnassus	5 (10)	3	4	0.96	0.511 ± 0.164	0.00281 ± 0.00137
	All samples	91 (182)	34	25	2.48	0.904 ± 0.011	0.00731 ± 0.00033
EF 1α	M. jurtina	28 (56)	25	24	1.85	0.881 ± 0.032	0.00183 ± 0.00021
	M. nurag	12 (24)	19	52	9.65	0.975 ± 0.021	0.00952 ± 0.00349
	M. megala	3 (6)	2	1	0.33	0.333 ± 0.215	0.00032 ± 0.00021
	M. cypricola	9 (18)	7	7	1.87	0.784 ± 0.085	0.00183 ± 0.00035
	M. chia	17 (34)	11	10	1.43	0.811 ± 0.052	0.00143 ± 0.00020
	M. telmessia	13 (26)	7	9	1.39	0.689 ± 0.088	0.00134 ± 0.00035
	M. halicarnassus	5 (10)	4	5	1.16	0.533 ± 0.180	0.00112 ± 0.00046
	All samples	87 (174)	53	66	2.72	0.851 ± 0.021	0.00285 ± 0.00060
mtDNA	M. jurtina	29	14	38	8.21	0.899 ± 0.036	0.01389 ± 0.00244
	M. nurag	9	5	26	12.61	0.861 ± 0.087	0.01974 ± 0.00250
	M. cypricola	12	10	51	17.59	0.955 ± 0.057	0.01740 ± 0.00519
	M. chia	6	4	35	12.13	0.867 ± 0.129	0.01159 ± 0.00688
	M. telmessia	11	7	54	11.45	0.873 ± 0.089	0.01052 ± 0.00570
	M. halicarnassus	8	6	13	4.39	0.893 ± 0.111	0.00403 ± 0.00106
	Lineage A	29	19	29	6.13	0.921 ± 0.041	0.00606 ± 0.00078
	Lineage B	51	17	37	9.63	0.904 ± 0.022	0.01776 ± 0.00153
	All samples	80	30	43	10.81	0.946 ± 0.011	0.01994 ± 0.00095
nDNA	M. jurtina	26 (52)	33	31	3.40	0.962 ± 0.015	0.00248 ± 0.00022
	M. nurag	11 (22)	21	29	6.41	0.996 ± 0.015	0.00457 ± 0.00029
	M. megala	3 (6)	4	8	3.93	0.867 ± 0.129	0.00276 ± 0.00059
	M. cypricola	9 (18)	11	13	2.93	0.908 ± 0.051	0.00208 ± 0.00039
	M. chia	14 (28)	20	14	3.33	0.974 ± 0.016	0.00245 ± 0.00021
	M. telmessia	13 (26)	16	12	2.61	0.902 ± 0.049	0.00186 ± 0.00030
	M. halicarnassus	5 (10)	5	9	2.11	0.756 ± 0.130	0.00148 ± 0.00071
	All samples	81 (162)	90	62	4.42	0.976 ± 0.005	0.00339 ± 0.00014