Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Endocr Relat Cancer. 2014 Nov 17;22(1):11–21. doi: 10.1530/ERC-14-0439

Figure 4.

Figure 4

Several miRs deregulated by signaling nodes that modulate rNIS-mediated RAIU in PCCl3 cells are predicted to bind to the 3′UTR of Nis. (A) Overexpression of miR-218a, miR-425, miR-96, miR-27b, or miR-539 did not result in a significant decrease (P=0.6529, 0.1834, 0.1777, 0.2638, and 0.9686 respectively) in TSH-induced rNIS-mediated RAIU activity in PCCl3 cells. (B) Overexpression of miR-195 resulted in a significant decrease in tRA/H-induced hNIS-mediated RAIU (30‰; P<0.0001) in MCF-7 cells. (C) Overexpression of miR-195 did not significantly decrease (P=0.2059) but overexpression of miR-182 and miR-494 did significantly decrease TSH-induced rNIS-mediated RAIU (27‰; P<0.0001 and 33‰; P<0.0001 respectively) in PCCl3 cells. (A, B and C) Oligo miRs were transfected into PCCl3 cells or MCF-7 cells for 24 h at the same time as TSH or tRA/H treatment respectively and then the cells were subjected to RAIU. The results are representative of two independent experiments performed in triplicates and the mean±s.d. for each set of results is shown.