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. Author manuscript; available in PMC: 2016 Feb 12.
Published in final edited form as: Neuroscience. 2014 Nov 26;0:87–96. doi: 10.1016/j.neuroscience.2014.11.030

Figure 6. Calsyntenin ectodomains mediate bead aggregation.

Figure 6

A. The ectodomain of each calsyntenin was fused to the Fc portion of human IgG and the CstnEC-Fc fusions were used in bead aggregation assays.

B–H. Each of the three Calsyntenin ectodomains, Clstn1EC-Fc (B, C, D), Clstn2EC-Fc (E, F, G) and Clstn3EC-Fc (H, I, J), are able to mediate calcium-dependent bead aggregation (n=3 for each condition). The aggregates formed by Clstn3EC-Fc (F) were smaller than those formed by either Clstn1EC-Fc (B) or Clstn2EC-Fc (D). Each fusion-protein also exhibits some calcium-independent aggregation (C, E, G). In each case, the calcium-dependent increase was statistically significant (p<0.05, Student's T-test). Scale bar = 50 µm.