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. Author manuscript; available in PMC: 2016 Feb 12.
Published in final edited form as: Neuroscience. 2014 Nov 26;0:87–96. doi: 10.1016/j.neuroscience.2014.11.030

Figure 7. Cadherin repeats are required for bead aggregation by Calsyntenin-1.

Figure 7

A. A series of domain deletions of the Clstn1EC-Fc were generated to test the role of the cadherin repeats, EC1 and EC2.

B–G. Deletion of EC1 (B,C), EC2 (D,E) or EC1–EC2 (F,G) abolish calcium-dependent and calcium-independent bead aggregation. Scale bar = 50 µm.

H, I. The cadherin repeats EC1–EC2 are sufficient to mediate calcium-dependent bead aggregation. There is also an increase in calcium-independent bead aggregation.

J. Deletion of either cadherin repeat is sufficient to abolish bead aggregation, while the two cadherin repeats are sufficient for bead aggregation (n=3 for each condition). The two cadherin repeats exhibit only a modest, but significant, calcium-dependence (p=0.04, Student's T-test).