FIG 2.

Automated (A) and conventional (B) sizing comparison with various DNA concentrations from 2 PCR products presenting 3 repeats on MIRU10. Lanes 1 to 8, sample 1 dilutions with DNA concentrations from 76 to 0.4 ng/μl. Lanes 9 to 11, sample 2 dilutions with DNA concentrations from 106 to 1.6 ng/μl. The expected size for all PCR products is 643 bp, and the accepted deviation is ±3.3%, i.e., 21 bp. The automated method was performed with a DNA high-resolution cartridge (OM1700 instrument method and alignment marker 15 to 3,000 bp), with an estimated PCR product consumption of <0.1 μl; conventional electrophoresis gel was performed loading 2 μl of PCR products on a NuSieve 3:1 3% gel with low-range-mass DNA ladder.