TABLE 1.
Summary of MIRU-VNTR allele sizes and distribution observeda
| MIRU-VNTR panel | Alias/locus | Size (bp) |
Allele distribution |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Minimum | Maximum | 0 | 1 | 2 | 3–3s | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 15 | Mixtureb | ||
| 15 locic (n = 82) | MIRU04/580 | 250 | 757 | 2 | 63 | 3 | 7 | 2 | 3 | 2 | ||||||
| MIRU10/960 | 590 | 961 | 8 | 48 | 17 | 2 | 3 | 2 | 1 | 1 | ||||||
| MIRU16/1644 | 565 | 738 | 1 | 4 | 19 | 57 | 1 | |||||||||
| MIRU26/2996 | 335 | 649 | 4 | 8 | 10 | 6 | 40 | 2 | 11 | 1 | ||||||
| MIRU31/3192 | 492 | 816 | 1 | 10 | 24 | 3 | 40 | 3 | 1 | |||||||
| MIRU40/802 | 398 | 630 | 8 | 9 | 42 | 10 | 12 | 1 | ||||||||
| Mtub04/424 | 602 | 784 | 1 | 25 | 12 | 38 | 6 | |||||||||
| ETR C/577 | 270 | 445 | 3 | 14 | 63 | 2 | ||||||||||
| Mtub21/1955 | 160 | 1,053 | 1 | 16 | 14 | 8 | 42 | 1 | ||||||||
| QUB-11b/2163b | 140 | 626 | 6 | 12 | 6 | 11 | 2 | 38 | 5 | 1 | 1 | |||||
| ETR A/2165 | 274 | 732 | 1 | 6 | 17 | 50 | 2 | 5 | 1 | |||||||
| Mtub30/2401 | 248 | 481 | 1 | 7 | 22 | 52 | ||||||||||
| Mtub39/3690 | 387 | 783 | 7 | 53 | 8 | 9 | 2 | 1 | 1 | 1 | ||||||
| QUB-26/4052 | 385 | 1,317 | 1 | 6 | 4 | 7 | 17 | 11 | 29 | 4 | 1 | 2 | ||||
| QUB-4156/4156 | 560 | 809 | 4 | 8 | 56 | 8 | 6 | |||||||||
| 24 locid (n = 66) | MIRU 02/154 | 456 | 513 | 3 | 62 | 1 | ||||||||||
| MIRU 20/2059 | 508 | 605 | 2 | 64 | ||||||||||||
| MIRU 23/2531 | 306 | 472 | 1 | 59 | 5 | 1 | ||||||||||
| MIRU 24/2687 | 439 | 505 | 59 | 7 | ||||||||||||
| MIRU 27/3007 | 644 | 665 | 65 | 1 | ||||||||||||
| MIRU 39/4348 | 584 | 755 | 1 | 17 | 47 | 1 | ||||||||||
| Mtub29/2347 | 451 | 579 | 1 | 7 | 58 | |||||||||||
| ETR B/2461 | 403 | 647 | 1 | 57 | 1 | 6 | 1 | |||||||||
| Mtub 34/3171 | 386 | 492 | 1 | 64 | 1 | |||||||||||
a
n = 1,824. Data were obtained from automated and manual methods. In case of noncongruence, Sanger sequencing was used as a reference method for accurate sizing of amplicons in order to infer the correct number of repeats to be retained.
b
Number of PCR products containing allele mixtures.
c
Sampling is 81 isolates and 1 reference strain.
d
Sampling is 65 isolates and 1 reference strain.