TABLE 1.

PCR primer sequences for amplification of the seven MLST housekeeping genes^a

Gene	Gene function	Amplification primer			Sequencing primer
		Sequence		Size (bp)	Sequence		Size (bp)
		Fwd	Rev		Fwd	Rev
atpA	ATP synthase alpha chain	GCGATTGCGAACTACACC	CTCGAGGAATACCTRACC	1,029	AGAAGGCGACGAAGTMAAGC	CRGAATCAGAAGCTGGWGCA	378
dnaE	DNA polymerase III alpha subunit	TGCGTCATCTGATTGAAA; C. ulcerans, TCCGAAACCTCATCGAGA	CGGTCCAATAAGACACCA; C. ulcerans, CAGTCCAATAAGAAACTA	858	GTGCGACAAGCTGGTGTG; C. ulcerans, GTGCGCCAAGCAGGTGTC	GGCTTWCGGCCATTYTTG; C. ulcerans, GGTTTACGGCCATTCTTG	354
dnaK	Chaperone protein DnaK	ACTTGGGTGGCGGTACTT; C. ulcerans, ACCTCGGCGGCGGAACCT	TGGTGAACGTCTCGGAAC; C. ulcerans, TGGTAAAGGTCTCAGAAC	696	AGATGGCTATGCAGCGTCT; same for C. ulcerans	GATGAGCTTGGTCATCACG; C. ulcerans, GATCAGCTTGGTCATCACG	345
fusA	Elongation factor G	TACCGCGAGAAGCTCGTT	GAAGGTTGGGTCCTCTTC	683	CGTAAGCTGACCGTTAACTC	CCATGGACTCRAGGATGA	360
leuA	2-Isopropylmalate synthase	CGTGCACTTCTACAACTC	ACCGTGATCGGTCTTCAT	865	CCYATCATCATCAAYCTGCC	CAGCTGGTTGCAGTAYTC	384
odhA	2-Oxoglutarate dehydrogenase	CGGCAAGGAAASCATGAC	GTTGTCGCCRAACATCTG	505	TBCAAGATCGCATYGARRC	TWGGCTCGATGTGKCCTTC	382
rpoB	RNA polymerase beta chain	AAGCGCAAGATCCAGGAC	TCGAACTCGTCGTCATCC	845	CGWATGAACATYGGBCAGGT	TCCATYTCRCCRAARCGCTG	342

^aThe dnaE and dnaK primer sequences specific for C. ulcerans are underlined; all other primers were the same for both C. diphtheriae and C. ulcerans.