TABLE 1.
Reproducibility of real-time PCR assay results
| Comparison | CV (%) with DNA copy no.a of: |
||||||
|---|---|---|---|---|---|---|---|
| 10 | 102 | 103 | 104 | 105 | 106 | 107 | |
| Within assayb | 0.9 | 0.5 | 0.2 | 0.4 | 1.2 | 1.5 | 0.5 |
| Between assaysc | 0.3 | 2.4 | 1.1 | 1.6 | 2.2 | 2.3 | 2.9 |
a
Tenfold dilutions of linearized plasmid DNA were used, numbers of copies per reaction mixture are shown, dilution series were thawed on 3 different days, and assays were performed in triplicate for each dilution.
b
Determined from three replicates within each assay.
c
Determined from three independent assays performed on different days.