TABLE 1.
Description of molecular platform and reagents
| Laboratory no. | Extraction method, manufacturer | Premix, manufacturer | Thermocycler, manufacturer |
|---|---|---|---|
| 1 | Manual, Qiagen | Sybr Ex Taq, TaKaRa | MX 3000, Stratagene |
| 2 | Manual, Qiagen | qPCR master mix, Promega | LightCycler 2.0, Roche |
| 3 | Automated, iPrep, Invitrogen | Sybr Ex Taq, TaKaRa | MX 3000, Stratagene |
| 4 | Automated, Easy Mag, bioMérieux | Sybr green master mix, Applied Biosystems | ABI 7900, Applied Biosystems |
| 5 | Automated, MagNA Pure Compact, Roche | Sybr Ex Taq, TaKaRa | SmartCycler, Cepheid |
| 6 | Manual, Qiagen | Sybr green master mix, Applied Biosystems | StepOne plus, Applied Biosystems |
| 7 | Manual, Qiagen | iQ Sybr green Supermix, Bio-Rad | Chromo4, Bio-Rad |