TABLE 1.
Primers used in the mPCR method for Aeromonas species identification
| Primer | Target organism (gene) | Sequence | Product (bp) | Primer concn (μM) |
|---|---|---|---|---|
| A-cav F(4) | A. caviae (gyrB) | 5′-TGCTGCTGACCATCCGC-3′ | 0.5 | |
| A-cav R(74) | 5′-GGTGCCTGCGGCTCG-3′ | 70 | 0.5 | |
| A-med F(176) | A. media (gyrB) | 5′-GGCCAAGCGTCTGCGT-3′ | 0.2 | |
| A-med R(275) | 5′-CGCCCTCGTAGCAGAAGTGA-3′ | 99 | 0.2 | |
| A-hyd F(533) | A. hydrophila (gyrB) | 5′-AGTCTGCCGCCAGTGGC-3′ | 0.45 | |
| A-hyd R(677) | 5′-CRCCCATCGCCTGTTCG-3′ | 144 | 0.45 | |
| A-Ver F(b1) | A. veronii (rpoB) | 5′-CGTGCCGGCTTTGAAGTC-3′ | 0.15 | |
| A-Ver R(b1) | 5′-GATCACGTACTTGCCTTCTTCAATA-3′ | 224 | 0.15 | |
| A-16S F(270) | Aeromonas | 5′-CGACGATCCCTAGCTGGTCT-3′ | 0.05 | |
| A-16S R(731) | Universal (16S rRNA gene) | 5′-GCCTTCGCCACCGGTAT-3′ | 461 | 0.05 |