FIG 1.

Analysis of the effect of depletion of anti-prM/E antibodies on NS1-MAC/GAC-ELISA using four different serotypes of antigens individually or in combination on three human sera confirmed as primary DENV-1 and DENV-2 and secondary DENV-2 infection, respectively. (A and B) P/N ratio values of IgM (A) and IgG (B) using a single serotype of VLPs for depletion and the same serotype of NS1 antigens for NS1-MAC-ELISA (both indicated on the x axis) using primary DENV-1 (left panel), primary DENV-2 (middle panel), and secondary DENV-2 (right panel) infection. (C) P/N ratio values of IgM and IgG using a combination of serotype 2 and 3 VLPs for depletion and the same combination of serotypes (2 and 3) of NS1 antigens for NS1-MAC-ELISA. P/N ratio without depletion of anti-E antibodies is shown by white bars, and that after depletion is shown by hatched bars. Normal human serum was used as a negative control to calculate the P/N ratio value by dividing the OD₄₅₀ of DENV-confirmed patient serum by that of negative-control serum. All data were obtained from three independent experiments, and standard deviations are indicated.