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. 2015 Jan 23;53(2):557–566. doi: 10.1128/JCM.02735-14

FIG 2.

FIG 2

Analysis of NS1-MAC/GAC-ELISA before and after depletion of anti-prM/E antibodies and comparison between VLP-MAC/GAC-ELISA and InBios DENV Detect IgM capture ELISA kit (InBios, Seattle, Washington) on 36 DENV-confirmed paired human sera collected during acute (≤14 days postonset) and convalescent (>14 days postonset) phases of infection. (A and B) Distribution of P/N values before and after depletion of anti-prM/E antibodies of 36 DENV-infected acute- (A) and convalescent-phase (B) sera by NS1-MAC-ELISA (left panels) and NS1-GAC-ELISA (right panels). (C and D) Correlations of P/N values obtained from acute- (C) and convalescent-phase (D) sera between VLP-MAC/GAC-ELISA and InBios DENV Detect IgM capture ELISA (upper left panel), VLP-MAC-ELISA and NS1-MAC-ELISA (upper right panel), InBios DENV Detect IgM capture ELISA and NS1-MAC-ELISA (bottom left panel), or VLP-GAC-ELISA and NS1-GAC-ELISA (bottom right panel). The regression line was added, and the Pearson correlation coefficient (r) was provided. A significance level α of 0.05 was obtained under a two-tailed P value. (E and F) The Bland-Altman plot of the difference of the VLP-MAC/GAC-ELISA and NS1-MAC/GAC-ELISAs' P/N ratio values was calculated with the average P/N ratio values from the two methods in the acute- (E) and convalescent-phase (F) sera. The solid black line indicates the bias value of a systematic difference between the two methods. The red dashed lines indicate 95% limits of agreement.