TABLE 1.

P/N ratio values obtained by using double, triple, or quadruple combinations of VLPs and NS1^a for NS1-MAC/GAC-ELISA on three confirmed DENV-infected patient sera

Combination of antigens	Absorptionb	P/N ratio value
		MAC-ELISA			GAC-ELISA
		Primary DENV-1	Primary DENV-2	Secondary DENV-2	Primary DENV-1	Primary DENV-2	Secondary DENV-2
1 + 2	−	1.218	1.270	1.206	1.248	1.138	1.078
	+	2.918	11.329	1.411	9.794	6.575	9.050
1 + 4	−	0.868	1.086	0.860	1.014	0.891	0.936
	+	1.854	2.490	1.324	2.338	1.644	2.004
2 + 4	−	1.337	1.039	1.033	0.872	0.786	0.830
	+	1.344	4.107	1.196	4.857	3.591	4.943
2 + 3	−	1.012	0.881	1.388	0.895	0.574	1.026
	+	2.133	6.941	1.202	8.126	4.323	9.175
1 + 3	−	0.884	0.751	1.299	1.187	0.998	1.125
	+	2.904	8.248	1.396	8.258	3.941	7.742
3 + 4	−	1.191	1.426	1.156	1.206	1.009	1.156
	+	2.192	4.711	1.464	3.615	2.044	3.655
1 + 2 + 3	−	0.824	0.695	0.736	0.913	0.809	0.938
	+	2.504	6.826	1.142	6.239	3.264	6.143
1 + 2 + 3 + 4	−	0.433	0.620	0.633	0.721	0.469	0.532
	+	1.414	3.175	0.722	3.242	2.249	3.148

^aSame serotype combination of VLPs and NS1 for NS1-MAC/GAC-ELISA.

^bAbsorption: +, sera incubated with VLPs before MAC/GAC-ELISA; −, sera without incubation with VLPs before MAC/GAC-ELISA.