TABLE 2.
Accuracy of MAC- and GAC-ELISA with use of VLP or sNS1 antigens
| Target panelb | Antigen(s) | AUC (95% CI) | % discriminatory probability (95% CI) |
|
|---|---|---|---|---|
| Sensitivity | Specificity | |||
| MAC-ELISA | ||||
| DENV | DENV-2 and -3 VLP | 0.990 (0.960–0.999) | 95.95 (88.6–99.2) | 100 (95.8–100) |
| DENV-2 and -3 NS1 | 0.976 (0.939–0.994) | 89.19 (79.8–95.2) | 95.35 (88.5–98.7) | |
| JEVa | JEV VLP | 0.944 (0.885–0.978) | 100.0 (79.2–100.0) | 88.9 (81.0–94.3) |
| JEV NS1 | 0.944 (0.885–0.978) | 100.0 (79.2–100.0) | 88.9 (81.0–94.3) | |
| WNVa | WNV VLP | 0.945 (0.896–0.976) | 92.8 (85.7–97.0) | 96.2 (87.0–99.4) |
| GAC-ELISA | ||||
| DENV | DENV-2 and -3 VLP | 0.937 (0.887–0.969) | 82.43 (71.8–90.3) | 90.7 (82.5–95.9) |
| DENV-2 and -3 NS1 | 0.936 (0.887–0.969) | 85.14 (75.0–92.3) | 88.37 (79.7–94.3) | |
| JEVa | JEV VLP | 0.933 (0.871–0.971) | 93.7 (69.7–99.0) | 92.9 (86.0–97.1) |
| JEV NS1 | 0.980 (0.934–0.997) | 100.0 (79.2–100.0) | 96.0 (90.0–98.9) | |
| WNVa | WNV VLP | 0.713 (0.634–0.784) | 46.4 (36.2–56.8) | 96.2 (87.0–99.4) |
| WNV NS1 | 0.673 (0.591–0.747) | 40.2 (30.4–50.7) | 94.3 (84.3–98.8) | |
a
Same protocol as DENV NS1-ELISA of using JEV- or WNV-VLP for depletion of prM/E antibodies and JEV- or WNV-NS1 for NS1-MAC/GAC-ELISA.
b
Control panels were other serocomplex panels and negative panels.