Figure 3.

Leptin fails to decrease the spontaneous firing rate or induce hyperpolarization of the RMP in ARH NPY neurons from DIO mice. A, B, Spontaneous APs from NPY neurons in acute brain slices from a lean, fasted mouse (A) or a DIO mouse (B). Leptin (100 nm) was added to the bath solution at the indicated time (arrow). The dotted line is at −65 mV. C, D, Summary bar graph of firing rate (C) and RMP (D). Statistical significance within dietary groups was determined using a paired Student's t test. All data presented as mean ± SEM.