Blockade of Kv channels prevents leptin-induced modulation of the intrinsic membrane properties of NPY neurons. A–C, Current-clamp recordings from an ARH NPY neuron in an acute brain slice from a fasted mouse in normal aCSF (A), with 100 nm leptin (B), and with 100 nm leptin and 20 mm 4-AP (C). Black trace, 0 pA; red trace, +10 pA stimulus; green trace, +30 pA stimulus. The dotted line indicates −60 mV. D, Stimulus–response curve for all three conditions. Data are presented as mean ± SEM.