Fig. 9.

Cells of subepicardium express a diverse composition of markers. Sections from 13 dpc hearts stained with anti-CD45 (b, l) (green), anti-CD68 (g), anti-Lyve1 (c, h) or anti-Fli1 (m) (red), anti-Flk1 (d, l, n) (white) antibodies; merged panels that include Hoechst staining of nuclei (blue) are visible in a, f, k, e, j, o. The areas located in white boxes of panels a, f, k are enlarged on panels e, j, o. Subepicardially located cells bearing Lyve1 antigen can co-express CD45 antigen (white arrow in e) or CD68 (blue arrow in j) or can be Lyve1/CD68/Flk1 triple-positive (red arrow in j). There are also cells bearing CD68 but not Lyve1 and Flk1 (yellow arrow in j). Some cells of the subepicardium are CD45/Fli1 double-positive and do not express Flk1 (green arrow in o). Scale bars 50 µm