Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 19;208(2):197–209. doi: 10.1083/jcb.201408024

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Shibuya et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Gls1 is not efficiently packaged into COPII vesicles. In vitro budding reactions were performed with microsomes from WT (CBY740) and gls1Δ (CBY1086) strains in the absence (−) or presence (+) of purified COPII components. Membranes from one tenth of the total reaction (T) and vesicle fractions were collected by centrifugation, resolved on 10.5% polyacrylamide gels, and immunoblotted for Gls1, Erv41, and Erv46 as well as the ER resident protein, Yet3, as a negative control.