Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 19;208(2):171–180. doi: 10.1083/jcb.201407077

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Otomo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Loss of lysosomal proteases and accumulation of storage material in B cells from MLII mice. (A) The activities of CtsB, CtsL, βHex, βGal, αFuc, and αMan in extracts of B cell blasts. Specific activities in B cells of WT mice were set to 100%. Mean and SEM (n = 3–6). (B) Western blot from B cell extracts. (C) Electron micrographs of splenic B cell blasts revealed accumulation of lysosomal storage material in MLII cells. Higher magnification of indicated area from a representative MLII B cell is shown. Bars, 1 µm. (D) Relative activities of CtsB, CtsL, βHex, βGal, αFuc, and αMan in extracts of T cell blasts (percentage of WT controls). Mean and SEM (n = 3–6). (E) Western blot from T cell extracts. m, mature form; p, precursor form. (F and G) Electron micrographs of purified splenic MLII CD4 T cells (F) and CD8 T cells (G) revealed normal appearance. Bars, 1 µm.