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. 2015 Jan 19;208(2):171–180. doi: 10.1083/jcb.201407077

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© 2015 Otomo et al.

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Figure 5. — Impaired antibody production in MLII mice. (A) Basal total IgM and IgG concentrations were determined in serum from WT and MLII mice. (B) Scheme of subcutaneous (s.c.) vaccination using 50 µg of Ova with complete Freund’s adjuvant (CFA) and blood analyses. (C and D) Ova-specific immunoglobulin titers were determined in mouse serum prior (pre) and on days 10 and 18 after immunization. Ova-specific IgM titers (C) and Ova-specific IgG, IgG1, and IgG2b (D) were determined by ELISA. The data are expressed as mean and SEM of five to seven experiments. A, absorbance units; ND, not detectable; *, P < 0.05; **, P < 0.01.