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. 2015 Jan 19;208(2):239–248. doi: 10.1083/jcb.201410042

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© 2015 Ducuing et al.

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Figure 3. — JNK and DPP form a coherent FFL that regulates cell differentiation. (A) Experimental design. The wild-type (WT) cell (black rectangle) secretes DPP (red dots) that induces its pathway in all cells (red nuclei). The absence of target (green) in the Prd>Bsk^DN cell abutting the wild-type cell indicates the presence of a JNK/DPP FFL. (B–C‴) Prd-Gal4, UAS-bsk^DN, Dpp-lacZ embryos marked for Jupiter::GFP (green in B; gray in B′) or Zasp52::GFP (green in C; gray in C′), phospho-Mad (red in B and C; gray in B″ and C″), and lacZ (blue in B and C; gray in B‴ and C‴). The brackets indicate the Bsk^DN domain, where DPP-lacZ (blue) is off. Anti–phospho-Mad (red) indicates that all cells receive DPP. Jupiter (B) and Zasp52 (C) in green are excluded from the Bsk^DN territory, even though DPP signaling is active (arrowheads), indicating that JNK acts also in parallel of DPP. (D–D″) Prd-Gal4, UAS-bsk^DN, Dpp-lacZ embryos marked for Jupiter::GFP (green in D; gray in D′) Jar (red in D; gray in D″) and lacZ (blue in D; gray in D‴). (E) Prd-Gal4, UAS-bsk^DN, Dpp-lacZ embryos marked for Zasp52::GFP and lacZ. All the markers are lost in the entire Bsk^DN territory (brackets in B–D or dotted lines in E). (F) Prd-Gal4, UAS-hep^ACT, Dpp-lacZ, Jupiter::GFP embryos marked for Jupiter::GFP (green in F; gray in F′), Jar (red in F; gray in F″), and lacZ (blue in F; gray in F‴). (G–H) Prd-Gal4, UAS-hep^ACT, Dpp-lacZ embryos marked for lacZ (magenta in G and H; gray in G″) and Zasp52 (green in G; gray in G′) or Zasp52::GFP (green in H). Ectopic JNK activity (dotted lines) induces Jar, Jupiter, and Zasp52 accumulation (arrowheads). Bars, 10 µm.