Figure 4.
Cytoskeletal components crucial for DC are also regulated by the JNK/DPP FFL. (A–H‴) Prd-Gal4, UAS-bskDN, Jupiter::GFP embryos (A–D‴) and Prd-Gal4, UAS-brk, Jupiter::GFP (E–H‴) marked for Jupiter::GFP (green in all panels; gray in A′–H′), α-tubulin (magenta in A and E; gray in A″ and E″) or actin (magenta in B, C, F, and G; gray in B″, C″, F″, and G″), or β-catenin (red in D and H; gray in D″ and H″) and E-Cadherin (blue in D and H; gray in D‴ and H‴). In all panels, the BskDN or the Brk overexpression territory is marked by the absence of Jupiter::GFP (brackets), and the border between the wild-type and the BskDN or Brk overexpression territory is delineated by the dotted lines. (I–N) Quantification of microtubule intensity, actin cable intensity, and filopodia numbers. Error bars: ±SEM (for all panels, Mann–Whitney’s U test: **, P < 0.01; ***, P < 0.001). bskDN or brk overexpression affects microtubules, β-catenin, and DE-Cadherin accumulation as well as actin cable formation at the LE and filopodia (arrowheads in C″ and G″). Bars, 10 µm.