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. 2015 Jan 19;208(2):239–248. doi: 10.1083/jcb.201410042

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© 2015 Ducuing et al.

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Figure 6. — The JNK/DPP FFL filters weak ectopic JNK activity. (A–C) Control (A), Puc^E69 (B), and Puc^E69, brk^M68 (C) stage 15 embryos stained for Jar, Jun, and Brk. Bars, 50 µm. (D) Quantification of Jar ectopic cells in the lateral epidermis. (n = 7; Mann–Whitney’s U test: **, P < 0.01.) Error bars: ±SEM. (E) Close-up of the lateral epidermis of a Puc^E69 embryo showing weak (red arrowheads) or strong (blue arrowheads) Jun expression. Bars, 10 µm. (F and G) Quantification of Jar expression in cells expressing low or high Jun levels in Puc^E69 versus Puc^E69, brk^M68 embryos. (F: two-way ANOVA and Bonferroni post-hoc test: ***, P < 0.001; G: Mann–Whitney’s U test: ***, P < 0.001.) Error bars: ±SEM. Brk represses Jar in about two thirds of the cells displaying weak Jun expression.