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. 2015 Jan 19;208(2):239–248. doi: 10.1083/jcb.201410042

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© 2015 Ducuing et al.

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Figure 7. — The JNK/DPP FFL canalizes LE specification and fosters DC robustness. (A–L) Control (top) and brk^M68 (bottom) embryos at 25°C (left) or 32°C (right) marked for Jar (yellow), Zasp52 (red), and Tubulin (green). Ectopic Jar, Zasp52, and microtubule accumulations are detected only in brk^M68 embryos at 32°C (arrowheads). Bars, 10 µm. (M) Quantification of Jar ectopic cells in control and brk^M68 embryos at 25°C or 32°C. Only brk^M68 embryos at 32°C exhibit Jar ectopic cells. n ≥ 7. Two-way ANOVA and Bonferroni post-hoc test: ***, P < 0.001. (N and N′) Width of the dorsal opening measured over time of control and brk^M68 embryos imaged at 25°C or 32°C. Only brk^M68 embryos at 32°C exhibit slower closure dynamics.