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. 2015 Jan 20;4:e04494. doi: 10.7554/eLife.04494

Figure 2. HOIL-1 is required in an innate immune cell compartment during Listeria infection.

(A) Survival of control and HOIL-1 KO reciprocal bone marrow chimeric mice following infection with 105 CFU Listeria. *p ≤ 0.0083; logrank Mantel–Cox test corrected for multiple comparisons. (B) Survival of RAG1 KO HOIL-1 WT (blue circles; n = 12) and RAG1 KO HOIL-1 KO (red squares; n=11) mice following infection with 104 CFU Listeria. (C) Listeria CFU in spleen and liver from RAG1 KO HOIL-1 WT (blue circles) and RAG1 KO HOIL-1 KO (red squares) mice infected with 104 CFU for 3 days. Each symbol represents an individual mouse and the mean log10 is indicated. For B and C, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; logrank Mantel–Cox test and Mann–Whitney test, respectively.

DOI: http://dx.doi.org/10.7554/eLife.04494.012

Figure 2.

Figure 2—figure supplement 1. Confirmation of hematopoietic reconstitution of bone marrow chimeric mice.

Figure 2—figure supplement 1.

Percent Hoil1/Rbck1+/+ (Hoil1/Rbck1 intron 7; top panel) and percent Hoil1/Rbck1−/− (neomycin-resistance cassette; bottom panel) genomic DNA (gDNA) in peripheral blood from control and HOIL-1 KO reciprocal bone marrow chimeric mice determined by qPCR.
DOI: http://dx.doi.org/10.7554/eLife.04494.013
Figure 2—figure supplement 2. HOIL-1 KO mice are capable of generating an adaptive immune response to Listeria.

Figure 2—figure supplement 2.

Listeria titers in spleen and liver of naïve (circles) or pre-immunized (103 CFU for 28 days, squares) control (blue symbols) and HOIL-1 KO (red symbols) mice challenged with 106 CFU Listeria for 3 days. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Statistical analyses were performed using two-way ANOVA with Holm-Sidak's multiple comparison test.
DOI: http://dx.doi.org/10.7554/eLife.04494.014