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. 2015 Jan 20;4:e04494. doi: 10.7554/eLife.04494

Figure 3. HOIL-1 is important for induction of pro-inflammatory cytokines following Listeria infection.

(A) TNFα (6 hr), IL-6 (6 hr) and IL-12p70 (24 hr) protein in macrophage culture supernatants following infection with Listeria (LM) ± IFNγ co-treatment. (B) Induction of Tnf, Il6 and Il12b transcripts in macrophages over 12 hr following infection with Listeria plus IFNγ. Data represent the mean ± SEM of macrophages derived from two mice per genotype analyzed in triplicate and are representative of at least three independent experiments. (C) Induction of cytokine transcripts in peritoneal cells over 12 hr following infection of control (blue circles) and HOIL-1 KO (red squares) mice with 105 Listeria. Each symbol represents an individual mouse. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Statistical analyses were performed using t-test.

DOI: http://dx.doi.org/10.7554/eLife.04494.015

Figure 3.

Figure 3—figure supplement 1. HOIL-1 is not required for listericidal activity of bone marrow-derived macrophages.

Figure 3—figure supplement 1.

Growth and killing of Listeria in untreated or IFNγ-pre-treated control and HOIL-1 KO macrophages at 0 and 6 hr post-infection. Data are from two independent experiments performed in duplicate.
DOI: http://dx.doi.org/10.7554/eLife.04494.016
Figure 3—figure supplement 2. Analysis of peritoneal cell populations following Listeria infection.

Figure 3—figure supplement 2.

Flow cytometric analysis of peritoneal cell populations in control (blue circles; 0 hr n = 20, 6 hr n = 10, 12 hr n = 7) and HOIL-1 KO mice (red squares; 0 hr n = 9, 6 hr n = 12, 12 hr n = 7) over 12 hr after infection with 105 CFU i.p. Data represent the mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Statistical analyses were performed using t-test.
DOI: http://dx.doi.org/10.7554/eLife.04494.017
Figure 3—figure supplement 3. HOIL-1 is important for induction of pro-inflammatory cytokines by innate cells following Listeria infection in vivo.

Figure 3—figure supplement 3.

Induction of cytokine transcripts in peritoneal cells from uninfected (0 hr) RAG1 KO HOIL-1 WT (blue circles) and RAG1 KO HOIL-1 KO (red squares) mice or 3 hr after infection with 104 Listeria. Each symbol represents an individual mouse.
DOI: http://dx.doi.org/10.7554/eLife.04494.018