TABLE 1.

Effect of AMP-DNM on ceramide and glycosphingolipids in 3T3-L1 adipocytes

Sphingolipid (pmol/well)	Ceramide	GM3	GM2
Without insulin
No addition	957 ± 71	2,192 ± 144	335 ± 21
With TNF	1,328 ± 95	3,265 ± 225	442 ± 20
With AMP-DNM	970 ± 57	1,063 ± 65	170 ± 12
With TNF + AMP-DNM	1,241 ± 88	2,205 ± 138	267 ± 18
With insulin
No addition	955 ± 56	2,451 ± 184	382 ± 26
With TNF	1,492 ± 92	4,288 ± 321	619 ± 36
With AMP-DNM	1,096 ± 56	1,074 ± 87	175 ± 10
With TNF + AMP-DNM	1,241 ± 99	1,988 ± 169	267 ± 97

Data are means ± SE. Serum-starved 3T3-L1 adipocytes were treated with either vehicle/control, AMP-DNM (10 μmol/l), and/or TNF-α (0.6 nmol/l) for 24 h prior to stimulation with or without insulin (100 nmol/l for 5 min). Sphingolipid content was determined by HPLC-based procedures as described in research design and methods. Values represent means of four wells. Similar results were obtained in two independent experiments.