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. 2014 Apr 22;40(4):489–501. doi: 10.1111/nan.12056

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© 2013 The Authors. Neuropathology and Applied Neurobiology published by John Wiley & Sons Ltd. on behalf of British Neuropathological Society.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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PtdIns(4,5)P2-positive structures corresponding to GVD bodies and NFTs. In the hippocampus of an AD case, the cellular localization of PtdIns(4,5)P2 was compared among haematoxylin and eosin (H&E)–stained sections (a,c), sections subjected to immunohistochemical analysis with 2C11 [b,d; some of the structures are GVD bodies (arrowhead) and some are NFTs (arrow)], sections incubated without primary antibody (e), and sections incubated with 10 mM neomycin sulphate/PBS prior to immunohistochemical staining with 2C11 (f). Scale bars: 20 μm.