Figure 1.
Number and baseline function of cancer patient DC. (a) MyDC (CD1c+) and plasmacytoid DC (pDC) (CD303+) were enumerated in heparinized whole blood from fully resected (n = 8, open circles) and Stage 4 (n = 9, filled circles) cancer patients using Countbeads to enable direct absolute quantification. Shown is cells × 104/ml for each patient and bar at mean for the group. (b) MyDC and moDC from healthy donors (n = 9, crosses), fully resected (n = 7 myDC, n = 6 moDC, open circles) and advanced cancer patients (n = 9 myDC, n = 8 moDC, filled circles) were stimulated in triplicate with poly I:C/R848 for 24 hr and cytokine production analyzed by ELISA. Shown is mean cytokine production for each donor with bar at median for the group.