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. 2013 Nov 20;134(3):575–586. doi: 10.1002/ijc.28398

Figure 3.

Figure 3

 The p38 MAPK plays a differential role in Th1 polarization by myDC and moDC in healthy donors. The effect of differential cytokine production on polarization of naïve Th responses was determined by co-stimulating naïve CD4+CD45RA+ T cells through CD3/CD28 in the presence of 25% supernatant from immature DC (Imm), DC treated with poly I:C/R848 (-p38i) or DC treated with p38 inhibitor SB203580 (10 µM) (+p38i) 1 hr prior to treatment with poly I:C/R848. Rested T cells were re-stimulated with αCD3/αCD28 for 20 h. (a) Flow cytometry plots of intracellular cytokine staining for IFNγ, IL-17 and IL-10 for two independent donors. Debris and dead cells were gated out using FS/SS and empty fluorescent channels prior to gating for IFNγ + (AF647), IL-10+ (PE) and IL-17+ (FITC). (b) Summary of % positive events for IFNγ, IL-17 and IL-10 for two donors for myDC (open bars) and moDC (filled bars). Dotted line shows the % positive events obtained following αCD3/αCD28 stimulation alone in the presence of blank medium without DC-derived cytokines.