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. 2013 Nov 20;134(3):575–586. doi: 10.1002/ijc.28398

Figure 5.

Figure 5

 Effect of melanoma lysate with or without p38 inhibitor on cytokine production by myDC from cancer patients. (a) MyDC (n = 7) from advanced cancer patients were treated in triplicate with p38 inhibitor SB203580 prior to poly I:C/R848. At 24 hr cytokine secretion was analyzed by ELISA. Shown is mean secretion of IL-12p70 and IL-10 for each patient plotted separately with poly I:C/R848 alone and with pre-treatment with MAPK inhibitor. (b) MyDC from fully resected (open circles, n = 7) and Stage 4 (filled circles, n = 8) cancer patients were treated for 1 hr with MM418 TDL before stimulation for 24 hr with poly I:C/R848. Cytokine secretion as a percentage remaining compared to stimulation without pre-treatment with TDL was calculated for each donor. (c) MyDC (individual symbols for each patient, n = 8) from advanced and fully resected cancer patients were treated as in (b) but with 1 hr pre-treatment with p38 inhibitor SB203580 prior to TDL treatment and TLR stimulation. Cytokine production (IL-12p70 and IL-10) for each condition for each donor is plotted. p < 0.05 (*) and p < 0.005 (**).