Skip to main content
. 2013 Dec 13;59(2):505–517. doi: 10.1002/hep.26659

Figure 1.

Figure 1

Low autophagic level and high miR-224 expression correlate with HBV-associated HCC. (A) Representative IHC of two paired HBV-associated HCC specimens showing the staining of Atg5, Beclin 1, and p62. MiR-224 staining was shown by miRNA in situ hybridization. Protein and miR-224 expression were determined by defining regions of interest (ROIs) using automated cell acquisition and quantification software (Histoquest). Arrow points to p62 accumulation. Scale bar = 20 μm. Quantification of the expression levels of Atg5, Beclin 1, p62, and miR-224 in 46 paired HBV-associated HCC specimens is shown as graphs. (B) Correlation of miR-224 expression with Atg5 and p62 was analyzed in 46 paired HBV-associated HCC specimens and linear regression coefficient and statistical significance are indicated. N, adjacent nontumor tissue; T, tumor tissue.