Figure 4.

MiR-224 is accumulated in the autophagosome but not let-7a. MiR-224 accumulation in the autophagosome was demonstrated by CQ blockage. Hep 3B cells were treated with amiodarone (5 μM) for 24 hours followed by CQ (50 μM) blockage for another 24 hours. The LC3 protein was labeled with green fluorescent tag. MiR-224 and let-7a were labeled with red fluorescent tags. The arrows point to colocalization of LC3 and miR-224 (A) or LC3 and let-7a (B). Scale bar = 10 μm. Quantification of colocalization was performed by counting 30 cells. *P < 0.05 and ***P < 0.001. N.T., nontreatment; CQ, chloroquine.