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. 2013 Dec 13;59(2):505–517. doi: 10.1002/hep.26659

Figure 7.

Figure 7

Liver tumors are suppressed by manipulation level of autophagy or miR-224 expression in an orthotopic rat model. In the former group, rat N1-S1 hepatoma cells were orthotopically injected into the rat livers, followed by intraperitoneal injection of DDW or amiodarone (30 mg/kg). In the latter group, rat N1-S1 cells were transfected with miR-224 antagonist (anti-miR-224) or negative control (anti-N.C), and injected into the rats’ livers. (A) Rats were sacrificed to collect the livers 1 week after orthotopic injection. The arrows point to tumors in the liver, and five mice were used in each group. Tumor formation was quantified by measuring the tumor weight. (B) Protein was extracted from the tumors of the former group, and the expression of LC3, Smad4 and β-actin was determined by western blotting. (C,D) Tumor sections of DDW (C) or amiodarone (D) treatment were further examined under TEM. Scale bar = 2 μm; White boxes in (C,D) are further enlarged. Scale bar = 500 nm. The arrows point the autophagosomes. N, nucleus. (E) The expression of miR-224 in the tumors after various treatments was evaluated by real-time PCR. Quantification of miR-224 expression is shown as mean ± SEM (n = 5). P values were obtained by Student t test. *P < 0.05 and **P < 0.01.