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. 2014 Jun 2;233(4):368–379. doi: 10.1002/path.4363

Figure 5.

Figure 5

miRNA-519a effects on viability and cell cycle. (a) Viability of MCF-7 WT cells was measured 48 and 72 h after transfection with miR-control or miRNA-519a. 7-AAD (b) and BrdU (c) staining was measured 72 h after transfection with miR-control or miRNA-519a to assay cell cycle phases. The S-phase population is gated in the red box for BrdU-positive cells. (d–f) Cell viability and cell cycle assays similar to a–c except that empty vector, pCMV-MIR or pCMV–MIR-519a overexpressing miRNA-519a were transfected as indicated. The results are presented as an average of two biological and three technical replicates, with significance levels explained in Figure 1.