Fig. 6. PYL8 interacts with and activates MYB44 and MYB73.
(A and B) PYL-MYB44 and PYL-MYB73 interactions in the yeast two-hybrid assay. Interaction was determined by yeast growth in media lacking His or His and Ade in the presence and absence of ABA. Dilutions (10−1, 10−2, and 10−3) of saturated cultures were spotted onto the plates, which were photographed after 5 days. (C) IAA19pro-LUC expression in aba2-1 mutant protoplasts cotransformed with the indicated combinations of PYL8, MYB44, MYB73, and IAA19pro-LUC with varying concentrations of ABA and IAA. n = 3 experiments. Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, Student's t test.