Skip to main content
. 2015 Jan 1;142(1):128–139. doi: 10.1242/dev.117887

Fig. 3.

Fig. 3.

Inhibition of BMP-mediated signaling pathways in early tooth germ by small inhibitor molecules. (A-E) Histological examination of E12.5 tooth germs after 4 days in organ culture in the presence of (A) DMSO, (B) SB203580, (C) U0126, (D) SB203580 and U0126 and (E) dorsomorphin. (F-O) Immunofluorescence of cyclin D1 (F-J) and BrdU labeling (K-O) of E12.5 tooth germs after 1 day in organ culture in the presence of inhibitors as indicated. (P-T) In situ hybridization of Pitx2 in E11.5 molar germs after 4 days in organ culture in the presence of inhibitors as indicated. Scale bars in A-T: 50 µm. (U-Y) Tooth-forming capability of E12.5 molar germs that were cultured for 4 days in vitro with each individual inhibitor prior to being subjected to subrenal culture for 10 days. Scale bars: 250 µm. (Z) Statistic comparison of BrdU-labeled cells in the dental epithelium after treatment with individual inhibitors. Error bars show mean±s.d.; **P<0.01, ***P<0.001.