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. 2014 Dec 18;15:27. doi: 10.1186/s12858-014-0027-0

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© Nayak et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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GST-DUSP5 and GST-S147P Proteins Purification. (A) GST-DUSP5 protein purification. Lane 1: MW marker, lane 2: cell lysate, lane 3: GST pull down (GST-DUSP5 was adsorbed on glutathione sepharose 4B and loaded on gel), lanes 4–7 purified GST-DUSP5 protein fractions. (B) GST-DUSP5 PreScission protease cleavage. Lane 1: MW marker, lane 2: Purified GST-DUSP5, lanes 3–6: GST-DUSP5 protein eluted following PreScission protease digestion. (C) GST-S147P protein purification. Lane 1: MW marker, lane 2: cell lysate, lane 3: GST pull down (GST-S147P was adsorbed on glutathione sepharose 4B and loaded on gel), lanes 4–7 purified GST-S147P protein fractions. (D) GST-S147P PreScission protease cleavage. Lane 1: MW marker, lane 2: purified GST-S147P, lane 3–6: protein eluted following PreScission protease digestion.